Work Package 2: Label-free SPRi detection of peptide microarrays
- Develop a device using localized Surface Plasmon Resonance Imaging (SPRi) on nanostructured surfaces to detect interaction kinetics with peptide microarrays.
- Develop software reading the HD-peptide array and calculating the kinetics constants for each spot. To make the software compatible with the IMS system.
The SPRi system is constructed from different components; each of them will be developed within the WP2. The work is constituted by the following tasks:
- development of an optics system
- fabrication of an electronics module and manual controls
- development of a microfluidics system and numerical simulation fluido-dynamics
- development of control software,
- development of reading software
These tasks will be carried out in parallel during the project. The components will be assembled in a bench-top prototype for laboratory use, which will then be used for the optimization and calibration of the HD-peptide arrays deposited over the nanostructured platforms. Finally an integrated, engineered computerized prototype will be developed.
- P4 is responsible for all SPRi development; P4 and several of the other partners will be responsible for designing and/or analysing peptide microarrays using SPRi.
- P5 for developing surface coatings
- P6 for developing 3D surfaces
- P7 for collaborating on integrating SPRi and MALDI instruments
- P3 for performing MALDI IMS analysis
- P10 for developing bioinformatics tools to analyse SPRi detections of peptide microarrays
- P1 for analysing immune reactions using peptide microarrays and SPRi read-out (i.e. understanding peptide-MHC stability)