Work Package 4: Label-free MOSFET detection of peptide microarrays
- Develop CMOS-based MOSFET arrays for the potentiometric detection of peptide interactions.
A first generation of arrays of 256 sensors will be used to validate the detection of peptide binding events by the technologies developed in WP2 and WP3 and based on the peptide immobilization on gold substrates processes developed in WP1. The expansion to arrays of 16,384 elements in a second generation of devices will be assessed
The development of MOSFET arrays will be divided into the following steps:
- Optimization of the CMOS gold electrodes, including the control and study of the effects of size, roughness and effective area. Study the electrochemical properties of gold-assembled peptides and their interaction with the analytes of interest.
- Design and fabricate a 16x16 array (256 elements) of MOSFETs with Au on the gate pads for potentiometric detection.
- Validate the performance of the devices against other techniques such as SPR, MALDI, and electrochemistry using known peptide/analyte systems.
- Design circuits that can be used for automation of array read-out.
- Assess the use of MOSFET arrays for measurement in Impedance mode as an alternative detection method.
Assess implementation of 128x128 arrays (16,364 elements).